Numerous studies conducted at different times and in various centers have shown that oxidative stress, mitochondrial dysfunction (insufficient ATP synthesis as an energy source), immune system dysregulation, and inflammation activation are commonly observed in autism. These studies measured the values of specific biomarkers in comparison with healthy control groups and performed comparative statistical analyses, identifying statistically significant differences. In these studies, comparative measurements were made on biopsy materials obtained from urine, blood samples, and brain tissues from the autism group and the healthy control group. Brain tissue samples are responsible for functions known to be impaired in autism (speech, social behavior, cognitive-learning-memory center, sensory and motor coordination center). Mainly; experimental autism modeling studies, human neuron cell culture studies, scientific studies conducted on children of different age groups diagnosed with autism. The results obtained from these studies have been published in numerous prestigious journals at regular intervals since 2010.

All these studies show that autism is a medical problem characterized by known behavioral disorder traits, stemming from clear biological foundations.